Purification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp.

Purification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp.

Two alpha-galactosidases (Ag-I & Ag-II) were purified from Acinetobacter sp. Both the enzymes were monomeric with pH optima of 7.0 and molecular weight of 65 kDa for Ag-I and 37 kDa for Ag-II. The temperature optima for Ag-I was between 50 and 60 °C and that of Ag-II was 40 °C. Both the enzymes were strongly inhibited by metal ions Ag2+ and Hg+, pCMB and SDS (1 %). The enzymes were found to be ...

Purification and Properties of Human a-Galactosidases*

The thermolabile oc-galactosidase (cy-galactosidase A) and thermostable a-galactosidase (cr-galactosidase B) were separated and purified from human placenta. A homogeneous a-galactosidase B preparation was obtained, but the a-galactosidase A preparation contained small amounts of contaminating protein and various other acid hydrolase activities. Each preparation had a molecular weight of approx...

Partial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)

Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through  (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...

Media Optimization, Purification and Characterization of Lipases from Acinetobacter Sp. Eh28 and Bacillus Subtilis Eh37

Partial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)

Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through  (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...